The sanction was handed to SK Slovan Bratislava after a number of supporter incidents during their Europa League play-off fixture against PAOK FC.
UEFA’s sanction means that Wolves supporters will not be able to attend the fixture on the 24th October, which will come as a major blow to fans hoping to follow the team during the club’s first European campaign in almost 40 years.
Wolves chairman Jeff Shi said: “Whilst we fully respect UEFA’s obligation to punish unacceptable behaviour by opposition supporters, we are deeply disappointed that this decision will ultimately affect our own fans and their ability to support the team.
“We have understandably explored all avenues to find a way in which to remedy this situation, but unfortunately we have been advised that there is no way around this decision."